Journal: Journal of Immunology Research

Article Title: OK-432 Acts as Adjuvant to Modulate T Helper 2 Inflammatory Responses in a Murine Model of Asthma

doi: 10.1155/2018/1697276

Figure Lengend Snippet: OK-432 is a strong Th1 adjuvant. (a) Schematic diagram depicting protocols for immunization protocol. The immune responses of B6 mice immunized with different formulations were determined. The levels of IFN- γ (b) and IL-4 (c) in culture supernatants, in which spleen cells were stimulated by 100 μ g/ml OVA for 6 days, and the titers of OVA-specific IgG2a (e) and IgG1 (f) in sera were measured by ELISA. The ratios of Th1/Th2 cytokine (d) and immunoglobulin (g) are shown. Data are represented as the mean ± SEM from two independent experiments ( n = 3–6 per group). ∗ p < 0.05; ∗∗ p < 0.01.

Article Snippet: Next, biotinylated rat anti-mouse IgG1 (A85-1), IgG2a (R19-15), and IgE (R35-118) monoclonal antibodies (all from BD PharMingen, San Diego, CA, USA) were added into the plates and incubated at 37°C for one hour.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Journal of Immunology Research

Article Title: OK-432 Acts as Adjuvant to Modulate T Helper 2 Inflammatory Responses in a Murine Model of Asthma

doi: 10.1155/2018/1697276

Figure Lengend Snippet: OVA/OK-432 formula induces more Th1 responses but controls Th2 reactions. The levels of IFN- γ (a), IL-4 (b), and IL-5 (g) in culture supernatants, in which spleen cells were restimulated by 100 μ g/ml OVA for 6 days in vitro, are shown. (c) IFN- γ /IL-4 ratios were calculated and depicted. The levels of OVA-IgG2a (d) and OVA-IgG1 (e) in sera were determined by ELISA. (f) OVA-IgG2a/OVA-IgG1 ratio from indicated mice was calculated and depicted. The titers of OVA-IgE (h) and total IgE (i) in sera from each group are shown. Data are represented as the mean ± SEM ( n = 8–12 per group) from three independent experiments. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001.

Article Snippet: Next, biotinylated rat anti-mouse IgG1 (A85-1), IgG2a (R19-15), and IgE (R35-118) monoclonal antibodies (all from BD PharMingen, San Diego, CA, USA) were added into the plates and incubated at 37°C for one hour.

Techniques: In Vitro, Enzyme-linked Immunosorbent Assay

Journal: NPJ Vaccines

Article Title: A Chlamydia trachomatis VD1-MOMP vaccine elicits cross-neutralizing and protective antibodies against C/C-related complex serovars

doi: 10.1038/s41541-021-00312-9

Figure Lengend Snippet: A/J mice were immunized 3 times s.c. with either 10 µg of extVD1 A *4/CAF01 or extVD1 J *4/CAF01. Three weeks after the last vaccination, sera from immunized mice were pooled ( n = 5–6), diluted 1:200, and the fine specificity of the IgG antibody responses was studied using a panel of biotinylated overlapping peptides (9-mers with 8 amino acid overlap) representing the extVD1 regions from SvA ( a ) and SvJ ( d ). Each bar represents the mean OD value of two determinations. Competitive peptide inhibition of in vitro neutralization of C.t . SvA and SvJ with peptides representing the four identified B-cell epitope regions in extVD1 A ( b ) and extVD1 J ( e ), respectively. Spleen cells were used to investigate the specific IFN-γ responses using panels of 20–22-mer peptides with 10 amino acid overlap (Supplementary Table ) spanning extVD1 A ( c ) and extVD1 J ( f ) regions. Cells from 6 mice/group were pooled and tested in triplicates. Each bar represents mean ± SEM. The individual experiment was repeated 2–3 times with similar results.

Article Snippet: After 2 h of incubation at room temperature, IFN-γ was detected by biotin-labeled rat anti-mouse IFN-γ (BD Pharmingen #554410) followed by Streptavidin-HRP both diluted in 1× PBS/1%BSA.

Techniques: Inhibition, In Vitro, Neutralization